Product Performance

ImmunoCAP Specific IgE test - the ideal immunoassay for allergen-specific IgE measurement

Although several commercially available in vitro options claim quantitative measurement of specific IgE, studies show results measured by ImmunoCAP systems are the most accurate, and are truly quantitative over the whole measuring range (1)

Characteristics of A Truly Quantitative Specific IgE Test

(2, 3, 4, 5)

Recovery of Antibodies - Excess of allergen minimizes interference by high levels of total IgE or other antibodies, and allows capture of important epitopes. Cross testing of calibrators and dilution recovery linearity tests in method comparison studies demonstrates test performance. Download (pdf) a CLSI/NCCLS-protocol based comparison study.
Parallelism -linearity upon dilution for reportable measuring rangeand down to the 0.1kUA/L limit of detection and quantitation.
Multipoint calibration curve traceable to the WHO reference preparation 75/502 for IgE Click here to view Curve Overlay
High Precision - With the fully automated ImmunoCAP instrumentation (ImmunoCAP 100E, ImmunoCAP 250, and ImmunoCAP 1000 systems), intra-assay CV's are less than 15% across the full measuring range regardless of allergen selected. Read more »

References

1. Precision and accuracy of commercial laboratories' ability to classify positive and/or negative allergen-specific IgE results. Szeinbach, SL, Barnes JH, Sullivan T, Williams PB. Ann Allergy Asthma Immunol. 2001 Apr;86(4):373-81

2. Usefulness of specific IgE antibody tests: a progress report. Williams PB. Ann Allergy Asthma Immunol. 2003 Dec; 91: 528-524

3. Quantitative IgE antibody assays in allergic diseases. Yunginger, et al. J Allergy Clin Immunol 2000 Jun;105(6 Pt 1):1077-78.

4. Understanding the usefulness of specific IgE blood tests in allergy. Ahlstedt S. Clin Exp All 2002;32 pp. 11-13.

5. ImmunoCAP Specific IgE 0-100 Directions for Use - U.S.